Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)
Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)
货 号：
GS13182
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
类 别：
Protein
别 名：
表达宿主：
Mammalian cell
种 属：
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
目录价格：
RMB
Data Sheet
MSDS
  • 产品详情
产品名称
Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)
货号
GS13182
蛋白构建
Uniorot ID:P0DTC2(319-541aa)
别名
表达宿主
Mammalian cell
种属
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
标签
N-terminal 10xHis-tagged and C-terminal Myc-tagged
纯度
Greater than 90% as determined by SDS-PAGE.
内毒素
Not test.
缓冲液
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
活性检测
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml. ③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 , the EC50 is 2.785-9.139 ng/ml. ④SARS-CoV-2 Spike protein RBD his/myc tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 13.8 nM as detected by LSPR Assay. ⑤Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated human ACE2 , the EC50 is 4.599-8.322 ng/ml.
存储
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
类别
Protein
用途
For research use only
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