Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)
Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)
货 号：
GS12882
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
类 别：
Protein
别 名：
NAD-ME, Malic enzyme 2
表达宿主：
E.Coli
种 属：
Homo sapiens (Human)
目录价格：
RMB
Data Sheet
MSDS
  • 产品详情
产品名称
Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)
货号
GS12882
蛋白构建
Uniorot ID:P23368(19-584aa)
别名
NAD-ME, Malic enzyme 2
表达宿主
E.Coli
种属
Homo sapiens (Human)
标签
C-terminal 6xHis-tagged
纯度
>95% as determined by SDS-PAGE and HPLC.
内毒素
Less than 1.0 EU/µg as determined by LAL method.
缓冲液
Lyophilized from a 0.2μm filtered PBS, pH 7.4.
活性检测
Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer . The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
存储
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
类别
Protein
用途
For research use only
上一篇:Recombinant Human Protein disulfide-isomerase protein(P4HB) (Active)下一篇:Recombinant Human Fructose-2,6-bisphosphatase TIGAR protein(TIGAR) (Active)

促销活动

相关产品