Recombinant Human Leukocyte surface antigen CD47(CD47),partial (Active)
Recombinant Human Leukocyte surface antigen CD47(CD47),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Leukocyte surface antigen CD47(CD47),partial (Active)
货 号：
GS13165
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
类 别：
Protein
别 名：
Antigenic surface determinant protein OA3（Integrin-associated protein）（IAP）（Protein MER6）（CD47）
表达宿主：
Mammalian cell
种 属：
Homo sapiens (Human)
目录价格：
RMB
Data Sheet
MSDS
  • 产品详情
产品名称
Recombinant Human Leukocyte surface antigen CD47(CD47),partial (Active)
货号
GS13165
蛋白构建
Uniorot ID:Q08722(19-139aa)
别名
Antigenic surface determinant protein OA3（Integrin-associated protein）（IAP）（Protein MER6）（CD47）
表达宿主
Mammalian cell
种属
Homo sapiens (Human)
标签
C-terminal hFc-tagged
纯度
Greater than 95% as determined by SDS-PAGE.
内毒素
Less than 1.0 EU/ug as determined by LAL method.
缓冲液
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
活性检测
①Measured by its binding ability in a functional ELISA. Immobilized SIRPA at 2 μg/ml can bind human CD47, the EC50 of human CD47 protein is 65.91-82.42 ng/ml. ②Human SIRPA protein His/Myc tag captured on COOH chip can bind Human CD47 protein Fc tag with an affinity constant of 19.1 nM as detected by LSPR Assay.
存储
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
类别
Protein
用途
For research use only
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