Recombinant Human Fibronectin(FN1),partial (Active)
Recombinant Human Fibronectin(FN1),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Fibronectin(FN1),partial (Active)
货 号:
GS13127
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
类 别:
Protein
别 名:
NovoNectin;Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
表达宿主:
E.coli
种 属:
Homo sapiens (Human)
目录价格:
RMB
  • 产品详情
产品名称
Recombinant Human Fibronectin(FN1),partial (Active)
货号
GS13127
蛋白构建
Uniorot ID:P02751(1270-1546aa & 1721-2016aa)
别名
NovoNectin;Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
表达宿主
E.coli
种属
Homo sapiens (Human)
标签
Tag-Free
纯度
Greater than 95% as determined by SDS-PAGE.
内毒素
Less than 1.0 EU/µg as determined by LAL method.
缓冲液
Lyophilized from a 0.2 μm Filtered 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 6.2
活性检测
Specific activity as determined by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells is greater than 50%. When 1×10^5 cells/well are added to plates coated with fibronectin ,approximately 50%-80% will adhere specifically after 30 minutes at 37℃.
存储
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
类别
Protein
用途
For research use only