Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)
Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)

Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)
货 号：
GS13193
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
类 别：
Protein
别 名：
表达宿主：
Mammalian cell
种 属：
Homo sapiens (Human)
目录价格：
RMB
Data Sheet
MSDS
  • 产品详情
产品名称
Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)
货号
GS13193
蛋白构建
Uniorot ID:Q9BYF1(18-740aa)
别名
表达宿主
Mammalian cell
种属
Homo sapiens (Human)
标签
C-terminal hFc-tagged
纯度
Greater than 85% as determined by SDS-PAGE.
内毒素
Less than 1.0 EU/ug as determined by LAL method.
缓冲液
Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
活性检测
①Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2μg/well of SARS-CoV-2 S1 protein , add 100μl/well of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. ②Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5μg/well of SARS-CoV-2 S1-RBD protein , add 100μl/well of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. ③Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5μg/well of SARS-CoV-2 S1-RBD protein , add 100μl/well of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. ④Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2μg/well of ACE2 protein, add 100μl/well of SARS-CoV-2 S1-RBD protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. ⑤The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. ⑥The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
存储
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
类别
Protein
用途
For research use only
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