Flag Monoclonal Antibody

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Western blot analysis of beta actin in COS7, Jurkat, C6 and 3T3 lysates using beta actin antibody (1:5000 diluted).

货号：

GS20002

类别：

Antibody

免疫原：

DDK tag,DYKDDDDK,Flag,Flag Tag

Immunofluorescence staining of 293T cells transfected with Flag tag by GS20002 at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L).

特异性：

DYKDDDDK

应用：

ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl

目录价格：

RMB

Western Blot: Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng; All lanes: Flag Tag antibody at 1:1000; Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.

Data Sheet

MSDS